I2PC – Instruct virtual course on Single Particle Analysis by CryoEM. Madrid, 18 – 20 April, 2022

Venue

Remote course
18 – 20 April, 2022 from 09.30-18.00 CET

Overall aims and course outline

Cryo-Electron Microscopy has established as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and it is capable of accessing to the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like PDB.

The aim of the course is to give an overall overview of the image processing in single particle analysis (SPA). The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.

For logistic reasons and travelling restrictions, the whole course will be given online. An additional benefit of this circumstance is that the course can be recorded and stay as a permanent course in I2PC. Although the course is online we will try to keep the interactions with attendees. For this reason, we will limit the maximum number of attendees to 40.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.

Expected impact for young researchers

CryoEM is the most rapidly growing structural technique. PhD students as well as young post-docs will certainly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Contacts

Lead contact:  
Carlos Oscar Sorzano
email. coss@cnb.csic.es

Administrative Contact:
Blanca Benitez
email: blanca@cnb.csic.es

Tutors

Carlos Oscar Sorzano Sánchez (COSS), José-María Carazo (JMC), Marcos Gragera (MG), Roberto Melero (RM), Daniel Marchán (DM), Estrella Fernández (EF), David Herreros (DH), Pablo Conesa (PC), Yunior Fonseca (YF)

Program

Day 1: Data processing

9:30       Morning:

  • Movie alignment, Screening micrographs and CTF
  • Particle picking and screening

13:00    Lunch Break

14:00    Afternoon:

  • Particle picking and screening
  • 2D classification
17:00    End of day

Day 2:

9:30       Morning:

  • Initial volume
  • 3D classification

13:00    Lunch Break

14:00    Afternoon:

  • 3D classification
  • 3D reconstruction
17:00    End of day
   

Day 3: 

9:30       Morning:

  • Validation and analysis

13:00    Lunch Break

14:00    Afternoon:

  • General discussion, open questions

17:00    End of day

* Virtual machines and I2PC staff will be available
until 17:30 for those who want to access.

REGISTRATION

Registration Fee: 50€ 
Fill  your application here (Regitration closed)

This course is organized by I2PC in collaboration with Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe and CRIOMECORR Project