Some pictures from the first and second days of the First Instruct Biennial Structural Biology Conference held in Heidelberg.
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Xmipp Workshop in Martinsried, March 12nd-13rd
The Workshop covers the details of the Xmipp software package that is used for reconstructing 3D maps from a set of randomly oriented particle images. The majority of the workshop consists of hands-on sessions designed to familiarize the attendees with the public-domain Xmipp software.
CTF Estimation Challenge
The CTF Estimation Challenge will be run from March 1st to July 15th as a collaboration between researchers, rather than a competition. Each challenger commits to submitting a manuscript to the next I2PC workshop summarizing the method used and results, and later to sharing the results with the community, such that we can foster a fruitful discussion at the meeting. Although several data sets will be made available, participants may choose to process only one data set if they prefer.
A paper summarizing all results will be prepared after the workshop.
We are pleased to announce the CTF estimation challenge called by the Madrid Instruct Image Processing Center (I2PC) in collaboration with the National Center for Macromolecular Imaging at Houston. By participating in this challenge, researchers will have the opportunity to test their ability estimating some of the key parameters of the Electron Microscope Contrast Transfer Function on a large micrograph data set produced by well-known laboratories on a wide set of experimental conditions.
FluoJ
Fluorescence microscopy image analysis is different for each sample but there are usually a common set of steps in the analysis workflow such as image preprocessing, segmentation, specimens characterization and classification.
In FluoJ user configures this steps for each sample and trains the classifier (optional) in order to do automatic image processing and classification. This way a variety of sample studies can be processed improving image analysis and minimizing user intervention. Contact us to try your images.
PeppeR new release: 1.11.02
A new version of the DAS client for 3D-EM, PeppeR, has been released. PeppeR is the annotation browser for macro-molecular structures that allows to display Hybrid models. These models are obtained by fitting the atomic cordinates derived from X-ray cristallograpgy or NMR; into the 3D volume maps obtained by Electron microscopy.
More details can be seen in the PeppeR home web. Try PeppeR v1.11.02, Now!
I2PC Developer Workshop
The first I2PC Developer Workshop will take place in Madrid, 6th and 7th of February. The main objective is to standardize information exchange in Single Particles as well as to start a dynamic of algorithm benchmarking in the field. Concrete conclussions on data interchange were reached and a first benchmarking challenge was issued to the community.
- Jose Maria Carazo
- Introduction and setting the goals and expectation of this meeting.
Benchmarks
- Wah Chiu
- Report on Map and Model Validation (from the Validation Task Force)
- Christian Blaschke
- Introduction to Bechmarking (I): The current situation in Bioinformatics
- Adrian Quintana
- Introduction to Bechmarking (II): The current situation in other imaging communities
- Anchi Cheng
- Presentation of Past Particle Picking Benchmark
- Steven Ludtke
- Presentation of Past Modeling Challenge
- Carlos Oscar Sorzano Sanchez
- Presentation of Past EU NoE ‘intended Benchmark’
- Adrian Quintana
- Presentation of I2PC Prototype Benchmark Platform
- Wah Chiu
- Discussion & Conclussions on Benchmark activities in 3DEM
Workflows in Single Particle
- Carlos Oscar Sorzano Sanchez
- Setting the scene
- Standardized Workflows
- Robert Langlois
- Workflows in Spider
- Roberto Marabini
- Workflows in XMIPP
- Steven Ludtke
- Workflows in EMAN
- Bernard Heymann
- Workflows in Bsoft
- Marin van Heel
- Workflows in Imagic
- Anchi Cheng
- Workflows in Appion
- Adrian Quintana
- Workflows in Scipion
- Jose Maria Carazo
- Discussion & Conclussions on Workflows
- Roberto Marabini
- Conclussions on Workflows
Ontologies and Vocabularies
- Roberto Marabini
- General presentation on Ontologies and information organization. Presentation of the Scipion Ontology
- John Westbrook
- Vocabularies and Ontologies. The case of the EMDB submission vocabulary
- Steven Ludtke
- Presentation of the EMEN Ontology (I & II
- Jose Maria Carazo & Roberto Marabini
- Discussion on Ontologies
3D Electron Microscopy Benchmark Web
3DEM Benchmark website, a joint initiative of the National Institute of Bioinformatics (INB) and the INSTRUCT Center for Image Processing in Microscopy, has been develop to foster the development of new advanced methods in the area of image processing in Structural Biology. To this end we provide a robust computational infrastructure capable of supporting the automatic and standardized benchmarking of image processing applications.