Scipion for facilities Workshop. October 30 – 31, 2025

Dates: October 30-31, 2025 

Venue: hybrid

National Center for Biotechnology (CNB), Madrid, Spain.
Madrid, October 30 – 31, 2025

Contact Us – I2PC – Instruct Image Processing Center

Aim:

The aim of this meeting is to gather feedback and showcase how different facilities are using Scipion, demonstrating its capabilities and benefits for Cryo-EM centers. The event will feature talks and ample time for discussion, aiming to share experiences with Scipion, discuss operational methodologies, and highlight new developments that can enhance our community’s daily workflow.

Conditions:

The workshop will be held on October 30 and 31 at the Spanish National Centre for Biotechnology (CNB) in Madrid, Spain. It can be attended either in person or remotely. No programming skills are required. We will use the CSIC Conecta video conferencing system for the online sessions — please verify your browser works at https://conecta.csic.es/.

Program:

We start the day at:

AGENDA

Thursday 30th October – Hybrid
10:00 – 10.30 Welcome and assistant’s introduction
10:30 – 11:00 An Unattended Image Processing Pipeline for On-the-Fly Quality Assessment and 3D Exploration in Cryo-EM – Daniel Marchán (CNB-CSIC, Madrid)
11:00 – 11:30 Deployment of Scipion in clusters (with and without containers)  – Lola Sánchez, Mikel Iceta and Irene Sanchez (CNB-CSIC, Madrid)
11:30 – 12:00 Coffee break
12:00 – 12:30

Mario Borgnia (NIEHS/NIH – Genome Integrity and Structural Biology)

12:30 – 13:00

Enhancing screening in acquisition time scipion-em-smartscope – Alberto García (CNB-CSIC, Madrid)

13:00 – 14:00 Lunch
14:00 – 14:30 nextPYP: a web-based platform for monitoring cryo-EM and cryo-ET sessions in real-time  – Alberto Bartesaghi (Duke University – Computer Science and Biochemistry)
14:30 – 15:00 The CryoEM CNB-CSIC facility setup: EMhub, iRODS, OSCEM – Lola Sánchez and Irene Sánchez (CNB-CSIC, Madrid)
15:00 – 15:30 Facilitating cryo-EM single-particle and tomography data analysis with EMhub- José Miguel (St. Jude Children’s Research Hospital, Memphis)
15:00 – 15:15 Discussion and conclusions
Friday 31st October – Hybrid
10.00 – 10:30 Live image processing with ScipionTomo for facilities – José Luis Vilas and Jorge Jiménez (CNB-CSIC, Madrid)
10:30 – 11:00 Balancing CryoSPA and CryoET: Building Skills and Innovation in a BSL-2 Facility – Carolin Seuring (Cryo-EM Technology Platform at CSSB Hamburg)
11:00 – 11:30 Four years of progress at the cryo-EM facility of Switzerland – Emiko Uchikawa (Dubochet Center for Imaging (DCI), Lausanne)
11:30 – 12:00 Using Scipion in SBGrid – Estrella Fernández (SBGrid Consortium, Boston)
12:00 – 12:30 Coffee break
12:30 – 13:00 Cryo-EM in CNPEM – Rodrigo Villares Portugal (CNPEM, Campinas)
13:00 – 13:30 Advancing Cryo-EM Services at ESRF with Scipion – Isai Kandiah (ESRF, Grenoble)
13:30 – 14:00 Discussion and conclusions
14:00 Lunch

Registration will be free for all attendeess

Further details, such as class locations, meeting links, title updates, and instructions for reaching the centre, will be provided in the weeks leading up to the workshop.

Scipion for facilities: Practical Course and Workshop. November 5th – 7th, 2024

Dates: November 5-7th 2024 (3 EU afternoons or 3 east US mornings)

Venue: Hybrid format

Aim: The aim of this meeting is to gather feedback and showcase how facilities are using Scipion, demonstrating its capabilities and benefits for Cryo-EM facilities. The event will feature talks and ample time for discussion, aiming to share experiences with Scipion, discuss operational methodologies, and highlight new developments that can enhance our community’s daily workflow.

Conditions: The workshop will be held at the Spanish National Centre for Biotechnology in Madrid, Spain. For the practical session on November 5, in-person attendance is required, and you must select this option on the registration page to ensure we prepare a machine for you. The main workshop on November 6-7 can be attended either in person or remotely.
No programming skills are required. We will use in-house bigbluebutton conference system. Verify your browser works at https://demo.bigbluebutton.org/

 

Program:

We start at:
San Francisco – 05:00 // Minneapolis – 07:00 // New York – 08:00 // São Paulo – 09:00 // Madrid – 14:00 // London – 13:00
Beijing – 20:00 // Tokyo – 21:00 // Sydney – 23:00

PRACTICAL COURSE

Tuesday 5th November – In person
14:00 – 14:30     Course Overview – Pablo Conesa, Biocomputing Unit CNB – Madrid, Spain.
14:30 – 15:30     On-the-fly processing – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
15:30 – 16:00     Coffee break
16:00 – 17:00     Processing workflows and templates – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
17:00 – 17:45     Scipion with queue systems – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
17:45 – 18:30     Build your own workflow – Support from CNB staff

 

WORKSHOP

Wednesday 6th November – Hybrid
14:00 (CET)      Welcome – Jose María Carazo , Biocomputing Unit CNB – Madrid, Spain.
14:15 – 14:45     An Overview of Scipion in Facilities – Pablo Conesa, Biocomputing Unit CNB – Madrid, Spain.
14:45 – 15:15     Automated on-the-fly data processing in EMhub is fantastic, but what’s next? – Jose Miguel de la Rosa Trevin, St Jude Children’s Research Hospital – Memphis, USA.
15:15 – 15:45     Lab Management with CEITEC: LIMS Demo and Key Features – Radek Veverka, Central European Institute of Technology at Masaryk University – Brno, Czech Republic.
15:45 – 16:15     Coffee break
16:15 – 16:45     Smart Data Collection with Smartscope – Jonathan Bouvette, Princeton University – New Jersey, USA.
16:45 – 17:15     Enhancing Smart Acquisition: Integrating SPA Processing Feedback with the Scipion-EM-SmartScope Plugin – Alberto Garcia, Biocomputing Unit CNB – Madrid, Spain.
17:15 – 18:00     Discussion and user input gathering
 
Thursday 7th November – Hybrid

14.00 (CET)      Enhancing Efficiency: Best Practices and Automations for On-the-Fly Processing in SPA Workflows – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
14:30 – 15:00     NextPyP: A scalable platform for single-particle cryo-EM/ET image analysis – Alberto Bartesaghi Duke University – Durham , USA.
15:00 – 15:30    Using Scipion to enable diverse cryo-ET workflows to explore Neurodegeneration at UCSF – Eric Tse, Institute for Neurodegenerative Diseases UCSF – San Francisco, USA.
15:30 – 16:00     Coffee break
16:00 – 16:30     Scipion use Case: Stream processing at CNB Facility –  Javier Chichon, CryoEM Facility CNB – Madrid, Spain.
16:30 – 17:00     Expanding access to cryo-EM through specimen preparation robotics and platform-independent remote imaging – Mario Borgnia, National Institute of Environmental Health Sciences – Durham, USA
17:00 – 18:00     Discussion and conclusions

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, November 5 – 7, 2024
How to find us: Contact Us – I2PC – Instruct Image Processing Center

REGISTRATION

Registration will be free for all attendees.
Fill your application here

Further details, such as class locations, meeting links, title updates, and instructions for reaching the centre, will be provided in the months leading up to the workshop.

Scipion at facilities workshop. October 17th & 18th, 2023

Dates: 17th & 18th October 2023 ( 2 EU afternoons or 2 US ET mornings)
Venue: Remote (https://conectaha.csic.es/b/bla-i7j-g7z-klh)

Aim: The aim of this meeting is to get feedback and showcase how facilities are using Scipion and show what Scipion offers to a Cryo-em facility. There will be talks but also plenty of time for discussion.

Target Audience: Cryo-em facility staff wanting to do or already doing on-the-fly processing.

Conditions: The workshop will be remote. No programming skills are required. We will use in-house bigbluebutton conference system. 
Verify your browser works at https://demo.bigbluebutton.org/

Program:

San Francisco – 05:00 // Minneapolis – 07:00 // New York – 08:00 // São Paulo – 09:00 // Madrid – 14:00 // London – 13:00
Beijing – 20:00 // Tokyo – 21:00 // Sydney – 23:00

Tuesday 17th October – New developments/Solutions

14.00 (CEST) Welcome – Jose María
14.15-14:40 Introduction to Scipion – Pablo Conesa (CNB) 
14:40-15:20 Optimizing Cryo-EM facilities with SmartScope – Jonathan Bouvette (Princeton)
15:20-16:00 Scipion and Smart scope – Alberto García (CNB)
16:00-16:40 EMhub: a web platform for CryoEM centers management and on-the-fly data processing (Jose Miguel de la Rosa (St. Jude Children’s Research Hospital Memphis, TN, USA)
17:20-18:00 Streaming and Unattended Workflows for SPA Processing – Daniel Marchan (CNB)

Wednesday 18th October – Facilities real cases

14.00 (CEST) Data flow in cryo-EM facilities: LMB example – Grigory Sharov (LMB)
14.15-14:40 CM01 and processing pipeline at ESRF – Isai and Olof (ESRF)
14:40-15:20 Setup at CNB – Javier Chichón (CNB)
15:20-16:00 Facilities + Tomography + Scipion – Pablo Conesa (CNB)
16:00-17:45 Discussion and conclusions 
             – Data storage and transfer
             – IT support
             – Reporting
             – Automation: Grid screening, acquisition image processing
             – Others? 
17:45-18.00 “Group picture” + End

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, October 17 – 18, 2023
How to find us: https://idospece.i2pc.margaro.eus/contact-us/

REGISTRATION

Registration will be free for all attendees.
Fill your application here

Scipion at facilities workshop. October 4th, 2022

Dates: 4th October 2022
Venue: Hybrid Meeting (Brno/Remote)
The face-to-face meeting will take place at the CEITEC facilities (Kamenice 753/5, Brno, 625 00).
CEITEC Website: https://www.ceitec.eu/

Aim : The aim of this meeting is to mainly get feedback and showcase how facilities are using Scipion and show what scipion offers to a Cryo-em facility. There will be talks but also plenty of time for discussion.

Target Audience: Cryo em facility staff wanting to do or already doing on-the-fly processing.

Conditions: The workshop will be hybrid:  online and presential (in Brno) for both attendees and speakers.
No programming skills are required.

Program: This program is tentative and may be subjected to changes depending on the attendees. Anyone is welcome to present their infrastructure.

9.00 Welcome
9.15 Brief update on Scipion for facilities CNB
9.45-13.00 Examples of setup, use, hardware and software architecture: ESRF, CNB, Francis Crick, SciLife Lab, NIEHS, UCSF (there will be a 20 minutes break)
13.00 Lunch
14.00 Discussion: Usability, maintainability, IT support, needs, reporting.
16.00 Brainstorming
16.45 Conclusions
17.00 End

REGISTRATION

Registration will be free for all attendees.
Fill  your application here

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I2PC Developer Workshop

The first I2PC Developer Workshop will take place in Madrid, 6th and 7th of February. The main objective is to standardize information exchange in Single Particles as well as to start a dynamic of algorithm benchmarking in the field. Concrete conclussions on data interchange were reached and a first benchmarking challenge was issued to the community.

Jose Maria Carazo
Introduction and setting the goals and expectation of this meeting.

Benchmarks

Wah Chiu
Report on Map and Model Validation (from the Validation Task Force)
Christian Blaschke
Introduction to Bechmarking (I): The current situation in Bioinformatics
Adrian Quintana
Introduction to Bechmarking (II): The current situation in other imaging communities
Anchi Cheng
Presentation of Past Particle Picking Benchmark
Steven Ludtke
Presentation of Past Modeling Challenge
Carlos Oscar Sorzano Sanchez
Presentation of Past EU NoE ‘intended Benchmark’
Adrian Quintana
Presentation of I2PC Prototype Benchmark Platform
Wah Chiu
Discussion & Conclussions on Benchmark activities in 3DEM

Workflows in Single Particle

Carlos Oscar Sorzano Sanchez
Setting the scene
Standardized Workflows
Robert Langlois
Workflows in Spider
Roberto Marabini
Workflows in XMIPP
Steven Ludtke
Workflows in EMAN
Bernard Heymann
Workflows in Bsoft
Marin van Heel
Workflows in Imagic
Anchi Cheng
Workflows in Appion
Adrian Quintana
Workflows in Scipion
Jose Maria Carazo
Discussion & Conclussions on Workflows
Roberto Marabini
Conclussions on Workflows

Ontologies and Vocabularies

Roberto Marabini
General presentation on Ontologies and information organization. Presentation of the Scipion Ontology
John Westbrook
Vocabularies and Ontologies. The case of the EMDB submission vocabulary
Steven Ludtke
Presentation of the EMEN Ontology (I & II
Jose Maria Carazo & Roberto Marabini
Discussion on Ontologies