Scipion for facilities: Practical Course and Workshop. November 5th & 7th, 2024

Dates: November 5-7th 2024 (3 EU afternoons or 3 east US mornings)

Venue: Hybrid format

Aim: The aim of this meeting is to gather feedback and showcase how facilities are using Scipion, demonstrating its capabilities and benefits for Cryo-EM facilities. The event will feature talks and ample time for discussion, aiming to share experiences with Scipion, discuss operational methodologies, and highlight new developments that can enhance our community’s daily workflow.

Conditions: The workshop will be held at the Spanish National Centre for Biotechnology in Madrid, Spain. For the practical session on November 5, in-person attendance is required, and you must select this option on the registration page to ensure we prepare a machine for you. The main workshop on November 6-7 can be attended either in person or remotely.
No programming skills are required. We will use in-house bigbluebutton conference system. Verify your browser works at https://demo.bigbluebutton.org/

 

Program:

We start at:
San Francisco – 05:00 // Minneapolis – 07:00 // New York – 08:00 // São Paulo – 09:00 // Madrid – 14:00 // London – 13:00
Beijing – 20:00 // Tokyo – 21:00 // Sydney – 23:00

PRACTICAL COURSE

Tuesday 5th November – In person

14:00 (CEST)      Welcome – Jose María Carazo , Biocomputing Unit CNB – Madrid, Spain.
14:15 – 14:30     Course Overview – Pablo Conesa, Biocomputing Unit CNB – Madrid, Spain.
14:30 – 15:40     On-the-fly processing – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
15:40 – 16:00     Coffee break
16:00 – 17:00     Processing workflows and templates – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
17:00 – 17:40     Scipion with queue systems – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
17:40 – 18:00     Discussion

 

WORKSHOP

Wednesday 6th November – Hybrid
14:00 (CEST)      Welcome – Jose María Carazo , Biocomputing Unit CNB – Madrid, Spain.
14:15 – 14:40     An Overview of Scipion in Facilities – Pablo Conesa, Biocomputing Unit CNB – Madrid, Spain.
14:40 – 15:20     Automated on-the-fly data processing in EMhub is fantastic, but what’s next? – Jose Miguel de la Rosa Trevin, St Jude Children’s Research Hospital – Memphis, USA.
15:20 – 16:00     Lab Management with CEITEC: LIMS Demo and Key Features – Radek Veverka, Central European Institute of Technology at Masaryk University – Brno, Czech Republic.
16:00 – 16:20     Coffee break
16:20 – 17:00     Smart Data Collection with Smartscope – Jonathan Bouvette, Princeton University – New Jersey, USA.
17:00 – 17:40     Enhancing Smart Acquisition: Integrating SPA Processing Feedback with the Scipion-EM-SmartScope Plugin – Alberto Garcia, Biocomputing Unit CNB – Madrid, Spain.
17:40 – 18:00     Discussion
 
Thursday 7th November – Hybrid

14.00 (CEST)      Enhancing Efficiency: Best Practices and Automations for On-the-Fly Processing in SPA Workflows – Daniel Marchan, Biocomputing Unit CNB – Madrid, Spain.
14:40 – 15:20     NextPyP: A scalable platform for single-particle cryo-EM/ET image analysis – Alberto Bartesagui Duke University – Durham , USA.
15:20 – 16:00     Use case: Title to be confirmed – Mario Borgnia, National Institute of Environmental Health Sciences – Durham, USA.
16:00 – 16:20     Coffee break
16:20 – 17:00     Use case: Title to be confirmed – Eric Tse, Institute for Neurodegenerative Diseases UCSF – San Francisco, USA.
17:00 – 17:40     ScipionTomo use Case: Tomo Streaming at CNB Facility –  Mikel Iceta & Javier Chichon, CryoEM Facility CNB – Madrid, Spain.
17:40 – 18:00     Discussion and conclusions

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, November 5 – 7, 2024
How to find us: Contact Us – I2PC – Instruct Image Processing Center

REGISTRATION

Registration will be free for all attendees.
Fill your application here

Further details, such as class locations, meeting links, title updates, and instructions for reaching the centre, will be provided in the months leading up to the workshop.

I2PC – Instruct-RI Hubs hybrid course on Electron Tomography and Subtomogram Averaging. Madrid, December 10 – 13, 2024

VENUE

Hybrid course.
December 10 – 13, 2024 from 16:00 to 21:30 CET.
You do not need to install any special software to connect. A modern browser should be enough.

Overall aims and course outline

By using image processing algorithms, it becomes possible to pinpoint the macromolecules of interest within the electron tomograms. Subsequently, researchers can extract small subtomograms from these locations and identify similar particles, facilitating their averaging and the recognition of diverse conformations. This process, referred to as subtomogram averaging, has played a pivotal role in elucidating an increasing number of macromolecular structures, achieving resolutions ranging from 20 to 3 A.

The course is designed to offer a comprehensive overview of the whole image processing workflow in Electron Tomography (ET). This includes tasks such as aligning the tilt series, reconstructing 3D tomograms, identifying particles, performing subtomogram averaging or even to perform a tomogram segmentation, all inside Scipion integration framework.  

With the experience gained in the organisation of virtual and in-person courses, we intend to organise this course in a hybrid format. We will be able to accommodate up to 20 individuals for in-person attendance. However, other 20 participants will attend remotely. In total, we can host 40 attendees. 

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures

Expected impact for young researchers

CryoET is one of the most promising structural techniques, and it bridges the gap between structural and molecular biology and cell biology. Ph.D. students, as well as post-docs, will undoubtedly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Prerequisites

  • You will need to bring a laptop with a modern browser
  • We will provide machines with the required software through Amazon Web Services.
  • We will provide launch and coffee breaks

Tutors: I2PC: Carlos Oscar Sorzano Sánchez, Jose-María Carazo, Pablo Conesa, David Herreros, Jorge Jiménez, Patricia Losana, Jose Luis Vilas, Ana Cuervo.
CryoEM CNB-CSIC: Francisco Javier Chichón.
Contacts: Lead contact:  Carlos Oscar Sorzano
E-mail: coss@cnb.csic.es
Administrative Contact: Blanca Benitez
E-mail: blanca@cnb.csic.es

Agenda

 Times are expressed in CET time Zone

DAY 1: 

Tomogram reconstruction

16:00 – Welcome(Jose Maria Carazo & Carlos Oscar Sorzano)
16:30 – Movie alignment and Tilt Series alignment: fiducidal and fiducialles approaches – (Jose Luis Vilas)
19:00- Break
20:00 – 3D Reconstruction, CTF estimation and correction(Jose Luis Vilas)
21:30 – End of session

DAY 2: 

Particle Picking methods

16:00 – Particle identification: different strategies, particle filtering according to their orientation in the tomogram– (David Herreros, Jorge Jiménez)
19:00- Break
20:00 – Particle identification: different strategies, particle filtering according to their orientation in the tomogram– (David Herreros, Jorge Jiménez)
21:30 – End of session

DAY 3: 

Subtomogram Averaging techniques

16:00 – Subtomogram averaging– (Ana Cuervo, Patricia Losada)
19:00- Break
20:00 – Particle filtering using strategies based on the subtomogram averaging and mapping back subtomograms(Ana Cuervo, Patricia Losada)
21:30 – End of session

DAY 4: 

Tomogram segmentation

16:00 – Tomogram data analysis and representation– (Javier Conesa, Javier Chichón, Jose Luis Vilas)
19:00 – End of course
16:00 – Discussion with the audience and short talks of the students work