iNEXT Discovery – I2PC Course on Single Particle Analysis by CryoEM. Madrid, 27 – 29 March, 2023


National Center for Biotechnology (CNB), Madrid, Spain
Madrid, March 27 – 29, 2023
How to find us:

Overall aims and course outline

Cryo-Electron Microscopy has established as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and it is capable of accessing to the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like PDB.

The aim of the course is to give an overall overview of the image processing in single particle analysis (SPA). The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.

With the experience gained in the organization of virtual and in-person courses, we plan to organize this course in an in-person modality. 20 people will be able to physically attend.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.

Expected impact for young researchers

CryoEM is the most rapidly growing structural technique. PhD students as well as young post-docs will certainly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.


Lead contact:  
Carlos Oscar Sorzano

Administrative Contact:
Blanca Benitez


Carlos Oscar Sorzano Sánchez (COSS), José-María Carazo (JMC), Marcos Gragera (MG), Carlos Perez Mata (CPM)


Day 1: Data processing
9:30 Morning session:
– Movie alignment, Screening micrographs and CTF
– Particle picking and screening
13:00 Lunch Break
14:00 Afternoon session:
– Particle picking and screening
– 2D classification
17:00 End of day

Day 2:
9:30 Morning session
– Initial volume
– 3D classification
13:00 Lunch Break
14:00 Afternoon session
– 3D classification advanced (masking, classification with no alignment…)
– 3D reconstruction
17:00 End of day

Day 3:
9:30 Morning session
– Sharpening, local resolution and validation
13:00 Lunch Break
14:00 Afternoon session
– One-to-one troubleshooting meeting. NEW
17:00 End of day


Registration will be free for all attendees.
Fill  your application here.  (Registration is closed)

This course is organized by iNEXT Discovery (UE Proposal:  871037) and I2PC in collaboration with  Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe and CRIOMECORR Project