News

Instruct course on flexibility analysis and integrative modelling using Scipion. Madrid, June 17 – 21, 2024

Venue

Madrid’s Institute for Material Sciences (ICMM-CSIC), Madrid, Spain
Madrid, June 17 – 21, 2024
How to find us: Contact | ICMM (csic.es)

Overall aims and course outline

Cryo-Electron Microscopy has established itself as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and is capable of accessing the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like the PDB.
The aim of the course is to introduce attendees to two techniques that are normally needed at the end of the Cryo-Electron Microscopy image processing pipeline: 1) understanding the possible continuous heterogeneity/flexibility of the sample, helping to obtain dynamical information in the form of conformational landscapes, and 2) interpreting the reconstructed map by means of an atomic model. The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.
We will use our software platform Scipion to present these two topics. Scipion is an integrative platform that allows interoperability among different software packages. In the domain of flexibility analysis, we include our own developments in Xmipp as well as tools coming from CryoDrgn, ProDy and HEMNMA. For the atomic modelling part, we include software like Coot, Chimera, Refmac, Phenix, and AlphaFold. In addition to simplifying the access to all these software packages in an integrated manner, Scipion adds a traceability and reproducibility layer so that any other researcher can understand the modelling workflow followed to reach a particular model.
This course is addressed to investigators at all levels.

Motivation for the I2PC course on conformational and compositional heterogeneity:
We want this course to help researchers to get introduced in the area of flexibility/heterogeneity analysis, one of the most advanced topics in SPA. For some of the most used methods in the field (some developed by us, some by others) we will
present the theory behind the method and perform a short practical. We will do so within the integrative Scipion Flexibility Hub environment, to help in the use of these multiple methods.

Expected impact for young researchers

Atomic modelling of the CryoEM map is currently at the moment the natural next step once the map is reconstructed. Flexibility analysis is currently not so common, but the advantage of CryoEM is that it can capture the different conformations of the macromolecules under study. Both techniques are valuable assets in young researchers already with a background on image processing in CryoEM.

Contacts

Lead contact:
Carlos Oscar Sorzano
email. coss@cnb.csic.es

Administrative Contact:
Blanca Benitez
email: blanca@cnb.csic.es

Tutors

Marta Martinez (MM) – Modelling specialist @BCU/i2PC
Roberto Marabini (RM) – Professor @UAM, Collaborator @BCU/i2PC
Carlos P. Mata (CM) – Single Particle Analysis specialist @BCU/i2pc
Marcos Gragera(MG) – Single Particle Analysis specialist @BCU/i2pc
David Herreros (DH) – Flexibility Hub head developer @BCU
James Krieger (JK) – Flexibility Analysis specialist @ BCU/i2PC
Carlos-Oscar Sorzano (COS) – Group leader @ BCU, Technical Director @ i2PC

Registration Fee

  • Academic Registration Fee Instruct countries: Predocs & Postdosc 50€
  • Academic Registration Fee Instruct countries: Others 100€
  • Academic Registration Fee Non Instruct countries: Predocs & Postdosc 100€
  • Academic Registration Fee Non Instruct countries: Others 150€
  • Industrial Registration Fee 300€

You can fill your registration here (closed)

Agenda

 Times are expressed in CET time Zone

DAY 1: Modeling

09:00-09:15 Welcome
09:15 – 10:15: Introduction to the compute environment to be used in this course (aws/scipion)
10:15 – 11:00:General introduction to model building in cryo-EM
11:00 – 11:30: Coffee break
11:30 – 13:00: Introduction to the data used in the practical session in homology modeling (details here) and de novo modeling
13:00-13:30: Map preprocessing I
13:30-14:30: Lunch break
14:30-16:00: Getting a first estimate of the model “de novo” (AlphaFold & ModelAngelo) and by sequence homology. 
16:00-16:30: Coffee break
16:30-17:00: Continuation of first estimation
17:00-17:30: Structure analysis: Structure comparison
17:30-18:00: Rigid Fitting of Initial Models
18:00: End of the day

DAY 2: Modelling

09:00-09:30: Review yesterday’s work 
09:30-11:00: Flexible fitting (Coot) and Validation
11:00-11:30: Coffee break
11:30-13:30: Flexible fitting and Validation
13:30-14:30: Lunch break
14:30-15:00: Review Kahoot students, teacher
15:00-16:00: Coffee break
16:30-17:00 Building the model of the whole protein
Chain merge & symmetrization
17:00-17:30: Submission of structures to EMDB. Understanding the validation report. Haemoglobin PDB full validation report 
Comparison validation scores PDB structure
17:30: Structure analysis: chain contacts Structural search
18:30:End of the day

DAY 3: Flexibility

9:30 – 10:00: Introduction to macromolecular flexibility and Flexibility Hub
10:00 – 10:15: Presentation of the dataset and workflows of the day (Zernike3D – HetSIREN)
10:15 – 11:00: Zernike3Deep (Theory)
11:00 – 11:30: Coffee break
11:30 – 12:30: Zernike3Deep (Practice)
12:30 – 13:00: HetSIREN (Theory)
13:00 – 14:00: Lunch break
14:00 – 15:00: HetSIREN (Practice)
15:00 – 15:30: Dimensionality reduction of estimated landscapes
15:30 – 16:00: Landscape exploration: interactive tools
16:00 – 16:30: Coffee break
16:00 – 17:00: Landscape exploration: interactive tools
17:00 – 17:30: End of the day

DAY 4: Flexibility

9:30 – 10:00: Review from the previous day
10:00 – 10:15: Presentation of the workflows of the day (FlexConsensus – ZART – CryoDRGN)
10:15 – 11:00: Should we trust our conformational landscapes? FlexConsensus (Theory)
11:00 – 11:30: Should we trust our conformational landscapes? FlexConsensus (Practice)
11:30 – 12:00: Coffee break
12:00 – 12:30: Should we trust our conformational landscapes? FlexConsensus (Practice)
12:30 – 13:00: Beyond motion blurr: ZART and flexible refinement (Theory)
13:00 – 14:00: Lunch break
14:00 – 15:00: Beyond motion blurr: ZART and flexible refinement (Practice)
15:00 – 15:30: CryoDRGN (Theory)
15:30 – 16:00: CryoDRGN (Practice)
16:00 – 16:30: Coffee break
16:30 – 17:00: CryoDRGN (Practice)
17:00 – 17:30: Landscape exploration: interactive tools
17:30 – 18:00: End of the day

DAY 5: Flexibility

9:30 – 9:45: Review from the previous day
9:45 – 10:00: Presentation of the workflows of the day (ContinuousFlex – 3DVA – 3DFlex – Prody)
10:00 – 11:00: ContinuousFlex
11:00 – 11:30: Coffee break
11:30 – 12:00: 3DVA (Theory)
12:00 – 13:00: 3DVA (Practice)
13:00 – 14:00: Lunch break
14:00 – 14:30: 3DFlex (Theory)
14:30 – 15:30: 3DFlex (Practice)
15:30 – 16:00: Prody
16:00 – 16:30: Coffee break
16:00 – 17:00: Prody
17:00 – 17:30: End of the day (closing remarks)

I2PC Course on Single Particle Analysis by CryoEM. Madrid, 20 – 22 March, 2024

Venue

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, March 20 – 22, 2024
How to find us: https://idospece.i2pc.margaro.eus/contact-us/

Overall aims and course outline

Cryo-Electron Microscopy has established as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and it is capable of accessing to the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like PDB.

The aim of the course is to give an overall overview of the image processing in single particle analysis (SPA). The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.

With the experience gained in the organization of virtual and in-person courses, we plan to organize this course in an in-person modality. 20 people will be able to physically attend.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.

Expected impact for young researchers

CryoEM is the most rapidly growing structural technique. PhD students as well as young post-docs will certainly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Tutors: I2PC: Carlos Oscar Sorzano Sánchez, Jose-María Carazo, Marcos Gragera, Carlos Perez Mata
CryoEM CNB-CSIC: Francisco Javier Chichón.
Contacts: Lead contact:  Carlos Oscar Sorzano
E-mail: coss@cnb.csic.es
Administrative Contact: Blanca Benitez
E-mail: blanca@cnb.csic.es

 

 

 

 

 

Agenda

 Times are expressed in CET time Zone

DAY 1: 9:30 Morning session:
– Movie alignment, Screening micrographs and CTF
– Particle picking and screening

13:00 Lunch Break

14:00 Afternoon session:
– Particle picking and screening
– 2D classification

17:00 End of day
DAY 2: 9:30 Morning session:
– Initial volume
– 3D classification

13:00 Lunch Break

14:00 Afternoon session:
– 3D classification advanced (masking, classification with no alignment…)
– 3D reconstruction

17:00 End of day
DAY 3: 9:30 Morning session:
– Sharpening, local resolution and validation

13:00 Lunch Break

14:00 Afternoon session:
– One-to-one troubleshooting meeting

17:00 End of day

Registration Fee

ACOMMODATIONS

REGISTRATION

The online registration will be open until  February 16th.
Fill your application here (closed). 

I2PC – Instruct-RI Hubs hybrid course on Electron Tomography and Subtomogram Averaging. Madrid, December 10 – 13, 2024

VENUE

Hybrid course.
December 10 – 13, 2024 from 16:00 to 21:30 CET.
You do not need to install any special software to connect. A modern browser should be enough.

Overall aims and course outline

By using image processing algorithms, it becomes possible to pinpoint the macromolecules of interest within the electron tomograms. Subsequently, researchers can extract small subtomograms from these locations and identify similar particles, facilitating their averaging and the recognition of diverse conformations. This process, referred to as subtomogram averaging, has played a pivotal role in elucidating an increasing number of macromolecular structures, achieving resolutions ranging from 20 to 3 A.

The course is designed to offer a comprehensive overview of the whole image processing workflow in Electron Tomography (ET). This includes tasks such as aligning the tilt series, reconstructing 3D tomograms, identifying particles, performing subtomogram averaging or even to perform a tomogram segmentation, all inside Scipion integration framework.  

With the experience gained in the organisation of virtual and in-person courses, we intend to organise this course in a hybrid format. We will be able to accommodate up to 20 individuals for in-person attendance. However, other 20 participants will attend remotely. In total, we can host 40 attendees. 

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures

Expected impact for young researchers

CryoET is one of the most promising structural techniques, and it bridges the gap between structural and molecular biology and cell biology. Ph.D. students, as well as post-docs, will undoubtedly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Prerequisites

  • You will need to bring a laptop with a modern browser
  • We will provide machines with the required software through Amazon Web Services.
  • We will provide launch and coffee breaks

Tutors: I2PC: Carlos Oscar Sorzano Sánchez, Jose-María Carazo, Pablo Conesa, David Herreros, Jorge Jiménez, Patricia Losana, Jose Luis Vilas, Ana Cuervo.
CryoEM CNB-CSIC: Francisco Javier Chichón.
Contacts: Lead contact:  Carlos Oscar Sorzano
E-mail: coss@cnb.csic.es
Administrative Contact: Blanca Benitez
E-mail: blanca@cnb.csic.es

Agenda

 Times are expressed in CET time Zone

DAY 1: 

Tomogram reconstruction

16:00 – Welcome – (Jose Maria Carazo & Carlos Oscar Sorzano)
16:30 – Movie alignment and Tilt Series alignment: fiducidal and fiducialles approaches – (Jose Luis Vilas)
19:00- Break
20:00 – 3D Reconstruction, CTF estimation and correction – (Jose Luis Vilas)
21:30 – End of session

DAY 2: 

Particle Picking methods

16:00 – Particle identification: different strategies, particle filtering according to their orientation in the tomogram– (David Herreros, Jorge Jiménez)
19:00- Break
20:00 – Particle identification: different strategies, particle filtering according to their orientation in the tomogram– (David Herreros, Jorge Jiménez)
21:30 – End of session

DAY 3: 

Subtomogram Averaging techniques

 16:00 – Subtomogram averaging– (Ana Cuervo, Patricia Losada)
19:00- Break
20:00 – Particle filtering using strategies based on the subtomogram averaging and mapping back subtomograms– (Ana Cuervo, Patricia Losada)
21:30 – End of session

DAY 4: 

Tomogram segmentation

16:00 – Tomogram data analysis and representation– (Javier Conesa, Javier Chichón, Jose Luis Vilas)
19:00 – End of course
16:00 – Discussion with the audience and short talks of the students work

 

I2PC – Instruct course on Cryo-Electron Tomography Image Processing. Madrid, December 12 – 15, 2023

VENUE

The course will take place in person at CNB-CSIC 
December 12 – 15, 2023 from 09:00 to 18:00 CET
You do not need to install any special software to connect. A modern browser should be enough.

Overall aims and course outline

Cryo-electron Tomography is a rapidly growing technique that can visualize cell organelles and biological macromolecules in their cellular context. Image processing algorithms allow the identification of the macromolecules of interest in the electron tomograms. Then, by extracting small subtomograms around those locations and identifying those similar particles, we may average them and recognize their different conformations. This latter process is called subtomogram averaging and has allowed solving a growing number of macromolecules with a resolution between 20-5A.

The course aims at providing a comprehensive overview of the whole image processing pipeline in Electron Tomography (ET) from the movie alignment of the tilt series, 3D reconstruction of the tomogram, particle identification, and subtomogram averaging, all inside Scipion integration framework.  

With the experience gained in the organization of virtual and in-person courses, we plan to organize this course in an in-person modality. 20 people will be able to physically attend.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.

Expected impact for young researchers

CryoET is one of the most promising structural techniques, and it bridges the gap between structural and molecular biology and cell biology. Ph.D. students, as well as post-docs, will undoubtedly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Prerequisites

  • You will need to bring a laptop with a modern browser
  • We will provide machines with the required software through Amazon Web Services.
  • We will provide launch and coffee breaks

Tutors: I2PC: Carlos Oscar Sorzano Sánchez, Jose-María Carazo, Pablo Conesa, Federico de Isidro,
David Herreros, Jorge Jiménez, Patricia Losana, Jose Luis Vilas, Borja Rodriguez,
CryoEM CNB-CSIC: Francisco Javier Chichón, Jose Javier Conesa.
Ramón y Cajal Researcher – Department of Information and Communication Engineering – University of Murcia: Antonio Martínez
Contacts: Lead contact:  Carlos Oscar Sorzano
E-mail: coss@cnb.csic.es
Administrative Contact: Blanca Benitez
E-mail: blanca@cnb.csic.es

Agenda

 Times are expressed in CET time Zone

DAY 1: 

From movies to tomograms

 9:30 – Welcome – (Jose Maria Carazo & Carlos Oscar Sorzano)
9:45 – Course introduction – (Jose Luis Vilas)
10:00 – Tomography in Scipion – (Jose Luis Vilas)
10:30 – Import Tilt Series, automatic alignment – (Federico de Isidro)
11:30 – 10 minutes break
11:40 – CTF estimation and correction –  (Federico de Isidro)
13:00 – Lunch break
14:00 – Tomogram reconstruction – (Federico de Isidro)
15:30 – 10 minutes break
15:40 – eTomo – (Grigory Sharov)
17:00 – AreTomo, ctfFind4 and novaCTF (Grigory Sharov)
17:30 – End of session

DAY 2: 

From tomograms to subtomograms- Picking and segmentation

9:30 – Non-directional picking with cryolo and SPA leap – (Borja Rodriguez)
11:10 – 10 minutes break
11:20 – Non-directional picking with cryolo and SPA leap – (Borja Rodriguez)
12:45 – Lunch break
13:45 – Tomogram denoising and membrane segmentation – (Antonio Martinez)
14:15 – Membrane picking with PySeg – (Jorge Jiménez)
15:00 – Dynamo – (Pablo Conesa)
16:00 – 10 minutes break
16:10 – Practice: Dynamo – (Pablo Conesa)
17:30 – End of session

DAY 3: 

Processing subtomograms

 9:30 –  Subtomogram averaging with RELION4 – (Patricia Losana)
11:30 – 10 minutes break
11:40 – Subtomogram Averaging with RELION4 – (Patricia Losana)
13:00 – Lunch Break
14:00 – Subtomogram Averaging with EMAN – (Jorge Jiménez)
15:00 – 10 minutes break 
15:10 – Subtomogram Averaging with EMAN – (Jorge Jiménez)
17:30 – End of session

DAY 4:  morning session

Tomogram Interpretation

 9:30 – Tomogram segmentation. Basic use of SuRVoS for cellular tomogram segmentation and basic analysis. 3D rendering using ChimeraUSFC with masked data – (Francisco Javier Chichón)
12:00/12:30 – End of coruse. Wrap Up.

 

Registration Fee

Fill your registration (Online registration closed)

 

This course is funded by Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe.

 

Scipion at facilities workshop. October 17th & 18th, 2023

Dates: 17th & 18th October 2023 ( 2 EU afternoons or 2 US ET mornings)
Venue: Remote (https://conectaha.csic.es/b/bla-i7j-g7z-klh)

Aim: The aim of this meeting is to get feedback and showcase how facilities are using Scipion and show what Scipion offers to a Cryo-em facility. There will be talks but also plenty of time for discussion.

Target Audience: Cryo-em facility staff wanting to do or already doing on-the-fly processing.

Conditions: The workshop will be remote. No programming skills are required. We will use in-house bigbluebutton conference system. 
Verify your browser works at https://demo.bigbluebutton.org/

Program:

San Francisco – 05:00 // Minneapolis – 07:00 // New York – 08:00 // São Paulo – 09:00 // Madrid – 14:00 // London – 13:00
Beijing – 20:00 // Tokyo – 21:00 // Sydney – 23:00

Tuesday 17th October – New developments/Solutions

14.00 (CEST) Welcome – Jose María
14.15-14:40 Introduction to Scipion – Pablo Conesa (CNB) 
14:40-15:20 Optimizing Cryo-EM facilities with SmartScope – Jonathan Bouvette (Princeton)
15:20-16:00 Scipion and Smart scope – Alberto García (CNB)
16:00-16:40 EMhub: a web platform for CryoEM centers management and on-the-fly data processing (Jose Miguel de la Rosa (St. Jude Children’s Research Hospital Memphis, TN, USA)
17:20-18:00 Streaming and Unattended Workflows for SPA Processing – Daniel Marchan (CNB)

Wednesday 18th October – Facilities real cases

14.00 (CEST) Data flow in cryo-EM facilities: LMB example – Grigory Sharov (LMB)
14.15-14:40 CM01 and processing pipeline at ESRF – Isai and Olof (ESRF)
14:40-15:20 Setup at CNB – Javier Chichón (CNB)
15:20-16:00 Facilities + Tomography + Scipion – Pablo Conesa (CNB)
16:00-17:45 Discussion and conclusions 
             – Data storage and transfer
             – IT support
             – Reporting
             – Automation: Grid screening, acquisition image processing
             – Others? 
17:45-18.00 “Group picture” + End

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, October 17 – 18, 2023
How to find us: https://idospece.i2pc.margaro.eus/contact-us/

REGISTRATION

Registration will be free for all attendees.
Fill your application here

I2PC Seminar Series 2023

Following the tradition, I2PC (Instruct Image Processing Center) is organizing the forth edition of a seminar series on cryoEM and cryoET methods for image processing. The aim of the seminar series is to gather expert developers and users in a same framework where the algorithms can gain visibility and users can understand the applications and mechanisms of the presented algorithms.

The webinars will be online and free without registration of cost. You can join the session through next link:
https://conectaha.csic.es/b/bla-rkh-dqa-rpn

The contents and speakers will be:

7th September (Time:  9:00 AM New York, 15:00PM Madrid, 14:00PM London). Bernard Heymann – The implications and resolution of the Ewald sphere issue in cryoEM. National Cryo-EM Program, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc

21th September (Time: 9:00AM Los Angeles, 18:00PM Madrid, 17:00PM London). Petar Petrov – The laser phase plate in cryo-EM: new approaches to image acquisition and analysis. Holger Müller group at UC Berkeley.

5th October (Time: 9:00AM New York, 15:00PM Madrid, 14:00PM London). Christopher JF Cameron – Reliable protein identification in cryo-EM images by machine-learning consensus.  Hemant Tagare and Mark Gerstein groups at Yale University.

19th October (Time: 9:00AM New York, 15:00PM Madrid, 14:00PM London). Eduard Baquero – Structural study of a broadly neutralizing anti-HIV-1 antibody from a post-treatment controller. NanoImaging Facility Core Institut Pasteur


Instruct course on flexibility analysis and integrative modelling using Scipion. Madrid, June 19 – 23, 2023

Venue

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, June 19 – 23, 2023
How to find us: https://i2pc.es/contact-us/

Overall aims and course outline

Cryo-Electron Microscopy has established itself as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and is capable of accessing the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like the PDB.
The aim of the course is to introduce attendees to two techniques that are normally needed at the end of the Cryo-Electron Microscopy image processing pipeline: 1) understanding the possible continuous heterogeneity/flexibility of the sample, helping to obtain dynamical information in the form of conformational landscapes, and 2) interpreting the reconstructed map by means of an atomic model. The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.
We will use our software platform Scipion to present these two topics. Scipion is an integrative platform that allows interoperability among different software packages. In the domain of flexibility analysis, we include our own developments in Xmipp as well as tools coming from CryoDrgn, ProDy and HEMNMA. For the atomic modelling part, we include software like Coot, Chimera, Refmac, Phenix, and AlphaFold. In addition to simplifying the access to all these software packages in an integrated manner, Scipion adds a traceability and reproducibility layer so that any other researcher can understand the modelling workflow followed to reach a particular model.
This course is addressed to investigators at all levels.

Expected impact for young researchers

Atomic modelling of the CryoEM map is currently at the moment the natural next step once the map is reconstructed. Flexibility analysis is currently not so common, but the advantage of CryoEM is that it can capture the different conformations of the macromolecules under study. Both techniques are valuable assets in young researchers already with a background on image processing in CryoEM.

Contacts

Lead contact:
Carlos Oscar Sorzano
email. coss@cnb.csic.es

Administrative Contact:
Blanca Benitez
email: blanca@cnb.csic.es

Tutors

I2PC staff: David Herreros (DH), James Krieger (JK), Marcos Gragera (MG), Marta Martínez (MM), Roberto Marabini (RM), Daniel del Hoyo (DDH)
CNRS IMPMC-CNRS UMR 7590. Sorbonne Université: Remi Vuillemot (RV)

You can fill your registration here (Online registration closed)

Registration Fee

  • Academic Registration Fee Instruct countries: Predocs & Postdosc 50€
  • Academic Registration Fee Instruct countries: Others 100€
  • Academic Registration Fee Non Instruct countries: Predocs & Postdosc 100€
  • Academic Registration Fee Non Instruct countries: Others 150€
  • Industrial Registration Fee 300€
Program
Day 1: June 19, 2023

09:30: Introduction to Scipion and AWS
10:15: Introduction to macromolecular flexibility and Flexibility Hub
10:30: Flexibility approaches (theory): 
– Deformation field-based approach
– Density-based approach
11:00: Coffee break
11:30: Presentation of the dataset to be used during the practical session
11:45: Estimation of conformational landscapes
12:45: Flexibility consensus (theory)
13:30: Lunch
14:30: Flexibility consensus (practice)
15:15: Dimensionality reduction of estimated landscapes
16:00: Coffee break
16:30: Conformational landscape analysis tools
– Interactive landscape clustering and real-time inspection of conformations
– Best number of clusters analysis
18:00 – 18:30: End of the day
Day 2 June 20, 2023

09:30: Review from the previous day
09:45: Conformational landscape analysis tools (continuation)
– Interactive landscape annotation and real-time inspection of conformations (2D-based tools)
11:00: Coffee break
11:30: Conformational landscape analysis tools (continuation)
– Interactive landscape annotation and real-time inspection of conformations (3D-based and hybrid tools)
13:30: Lunch
14:30: Extracting conformational states from landscape selections:
Application of deformation fields to maps and structural models + strain/rotation analysis + motion statistics
15:00: Interactive local analysis of motions
15:30: Heterogeneity correction with ZART (theory)
16:00: Coffee break
16:30: Heterogeneity correction with ZART (practice)
17:15: Heterogeneity correction of different landscapes states with ZART
18:00 – 18:30: End of the day
Day 3 June 21, 2023

09:00: Review from the previous day
9:45: Introduction to atomic structure ensemble analysis, normal mode analysis, and ProDy
10:15: Ensemble analysis with ProDy ensemble construction, PCA and landscape projection, and motion analysis with NMWiz
11:00: Coffee break
11:30: Conformational landscapes from normal modes and molecular dynamics using ContinuousFlex (theory and practice)
13:30: Lunch
14:30: Introduction to model building in cryo-EM
  – How to get a first estimation of the model (homology, alphafold, modelangelo)
15:15: Presentation of the data used in the practical session
– Kahoot
15:45 min: Map preprocessing – I
  16:00: coffee break
16:30: Map preprocessing – II
17:00 : Getting a first estimate of the model
18:00-30: End of the day
Day 4 June 22, 2023

09:30 Structure analysis: Structure comparison
10:15: Rigid fitting of initial models
11:00: Coffee Break
11:30: Flexible fitting (coot) and validation (phenix)
13:30: lunch
14:30: Flexible fitting (coot) and validation (phenix) (cont)
15:30: kahoot
16:00: coffee break
16:30: Model building of the Beta sub-unit
18:00-30: end of the day
Day 5 June 23, 2023

09:30: Presentation of the course questionnaire
09:45: Building the model of the protein complex
10:30: kahoot.
11:00: Coffee Break
11:30: Submission of structures to EMDB. Understanding the validation report.
12:00: Structure analysis: Chain contacts
13:00: INSTRUCT short message and farewell
13:30: lunch and end of the day

Fill your registration here (Online registration closed)

This course is funded by Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe.

iNEXT Discovery – I2PC Course on Single Particle Analysis by CryoEM. Madrid, 27 – 29 March, 2023

Venue

National Center for Biotechnology (CNB), Madrid, Spain
Madrid, March 27 – 29, 2023
How to find us: https://i2pc.es/contact-us/

Overall aims and course outline

Cryo-Electron Microscopy has established as a mature technique to determine the three-dimensional structure of biological macromolecules. It is currently reaching atomic resolution and it is capable of accessing to the structure of conformationally heterogeneous samples, as well as macromolecular complexes. It is complementary to other structural techniques like X-ray crystallography and Nuclear Magnetic Resonance and at the moment it is the most rapidly growing technique in atomic structure depositions at public databases like PDB.

The aim of the course is to give an overall overview of the image processing in single particle analysis (SPA). The I2PC has a long tradition of organizing courses of image processing and atomic modelling, with Instruct courses that have been heavily oversubscribed and had to be repeated twice in the same year to be able to attend its demand.

With the experience gained in the organization of virtual and in-person courses, we plan to organize this course in an in-person modality. 20 people will be able to physically attend.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.

Expected impact for young researchers

CryoEM is the most rapidly growing structural technique. PhD students as well as young post-docs will certainly benefit from approaching this technique. Knowing it will open new doors to new positions worldwide.

Contacts

Lead contact:  
Carlos Oscar Sorzano
email. coss@cnb.csic.es

Administrative Contact:
Blanca Benitez
email: blanca@cnb.csic.es

Tutors

Carlos Oscar Sorzano Sánchez (COSS), José-María Carazo (JMC), Marcos Gragera (MG), Carlos Perez Mata (CPM)

Program

Day 1: Data processing
9:30 Morning session:
– Movie alignment, Screening micrographs and CTF
– Particle picking and screening
13:00 Lunch Break
14:00 Afternoon session:
– Particle picking and screening
– 2D classification
17:00 End of day

Day 2:
9:30 Morning session
– Initial volume
– 3D classification
13:00 Lunch Break
14:00 Afternoon session
– 3D classification advanced (masking, classification with no alignment…)
– 3D reconstruction
17:00 End of day

Day 3:
9:30 Morning session
– Sharpening, local resolution and validation
13:00 Lunch Break
14:00 Afternoon session
– One-to-one troubleshooting meeting. NEW
17:00 End of day

REGISTRATION

Registration will be free for all attendees.
Fill  your application here.  (Registration is closed)

This course is organized by iNEXT Discovery (UE Proposal:  871037) and I2PC in collaboration with  Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe and CRIOMECORR Project


Scipion at facilities workshop. October 4th, 2022

Dates: 4th October 2022
Venue: Hybrid Meeting (Brno/Remote)
The face-to-face meeting will take place at the CEITEC facilities (Kamenice 753/5, Brno, 625 00).
CEITEC Website: https://www.ceitec.eu/

Aim : The aim of this meeting is to mainly get feedback and showcase how facilities are using Scipion and show what scipion offers to a Cryo-em facility. There will be talks but also plenty of time for discussion.

Target Audience: Cryo em facility staff wanting to do or already doing on-the-fly processing.

Conditions: The workshop will be hybrid:  online and presential (in Brno) for both attendees and speakers.
No programming skills are required.

Program: This program is tentative and may be subjected to changes depending on the attendees. Anyone is welcome to present their infrastructure.

9.00 Welcome
9.15 Brief update on Scipion for facilities CNB
9.45-13.00 Examples of setup, use, hardware and software architecture: ESRF, CNB, Francis Crick, SciLife Lab, NIEHS, UCSF (there will be a 20 minutes break)
13.00 Lunch
14.00 Discussion: Usability, maintainability, IT support, needs, reporting.
16.00 Brainstorming
16.45 Conclusions
17.00 End

REGISTRATION

Registration will be free for all attendees.
Fill  your application here. 

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I2PC – INSTRUCT COURSE: INTRODUCTION TO MODEL BUILDING

Venue

Remote course
June 22th to June 24th (2022) (3 days):
*3 Mornings: Presentations and training.
*2 Afternoons: Personal work.

Aim of the course:

Hands-On Training Course that introduces the  fundamentals and main tools needed to build an atomic model in the context of cryo-EM, starting from a 3D map and the aminoacid sequence of each protein chain. The  tools will be used inside the Scipion framework (de la Rosa-Trevin et al. 2016) and examples will focus on the modeling by homology scenario, including AlphaFold2 prediction models.

Expected impact for young researchers

Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM structure determination process. In this course, we introduce the basic tools needed to perform this task. Ph.D. students, as well as post-docs, will undoubtedly benefit from approaching this technique since atomic models guide our mechanistic interpretation of cryo-EM structures.

Contacts

Lead contact:  
Carlos Oscar Sorzano
email. coss@cnb.csic.es

Administrative Contact:
Blanca Benitez
email: blanca@cnb.csic.es

Tutors

Marta Martínez (MM), Roberto Marabini (RM)

Schedule and contents:

*June 22th:

  • 9:30: Welcome and presentation of the course, dynamics and mentors.
  • 10:00: Introduction to model building in the context of cryo-EM and Scipion
  • 10:30: Introduction to Scipion (import data)and AWS
  • 11:30: Map preprocessing
  • 12:00: Break
  • 12:15: Introduction to AlphaFold2 (Searching for prediction models)
  • 13:00: Searching models by homology
  • 13:30: Afternoon task proposal

*June 23th:

  • 9:30: Review of contents and follow up of the afternoon task proposal.
  • 10:00: Kahoot
  • 10:15: Rigid fitting of initial models
  • 11:00: Flexible fitting
  • 12:00: Break
  • 12:15: Flexible fitting (continuation)
  •  14:00: Afternoon task proposal

*June 24th:

  • 9:30: Review of contents and follow up of the afternoon task proposal.
  • 9:45: Presentation of the course questionnaire
  • 10:00: Kahoot
  • 10:15: Assessment and validation of models
  • 11: 00: Building the model of the protein complex
  • 11:15: Break
  • 11:30: Structure analysis: Chain contacts
  • 12:00: Structure analysis: Structure comparison
  • 12:30: Submission of structures
  • 13:00: Summary
  • 13:30: INSTRUCT short message and farewell

REGISTRATIONRegistration Fee: 50€ 
Registration online closed.

This course is organized by I2PC in collaboration with Instruct – ERIC, an Integrated Structural Biology Infrastructure for Europe and CRIOMECORR Project